SPD-20A/ 20AV Series
Prominence HPLC UV-Vis Detectors
Part of the SPD-20 Series, which also includes the SPD-M20A Photo Diode Array Detector, the SPD-20A/ 20AV UV-Vis detectors offer an exceptional level of sensitivity and stability. Taking sensitivity to the limit, the SPD-20A/ 20AV has a noise level of 0.5× 10-5 AU max., making it the world' s most sensitive UV-Vis detector ( ASTM Standards) . One mode of the SPD-20AV allows both the deuterium and tungsten lamps to be lit simultaneously, enabling high-sensitivity wavelength-programming detection for ultraviolet light and the entire visible-light range.
Greater Baseline Stability
Supplied as standard, a temperature-controlled flow cell eliminates inconsistencies caused by changes in absorbance due to shifts in room temperature. This helps increase the baseline stability and the analysis reliability.
Superior Linearity - 2.5AU
Using newly developed signal processing technology, the stray-light correction function has been enhanced, and the linearity has been improved. This expanded linearity combines with various noise-reduction technologies to provide users with a wide dynamic range and allow for the analysis of your target compound and minor impurities in a single run.
Internal Validation Protocol
The incorporation of a low-pressure mercury lamp for wavelength calibration ensures simple calibration in the ultraviolet region.
Monitor two components in the UV or VIS range simultaneously
Simultaneous D2 and W lamp Illumination
Available with the SPD-20AV, users can eliminate warm-up time and baseline disturbances during wavelength programming between the UV and Visible range. With both lamps lit, high-sensitivity analysis throughout the full wavelength spectrum is ensured.
Light source Deuterium ( D2) lamp Deuterium ( D2) lamp, tungsten ( W) lamp
Number of diode elements None
Wavelength range 190 to 700 nm 190 to 900 nm
Bandwidth, slit width 8 nm
Wavelength accuracy 1 nm max.
Wavelength precision 0.1 nm max
Noise 0.5 X 10-5 AU ( under specified conditions)
Drift 1 X 10-4 AU/ h ( under specified conditions)
Linearity 2.5 AU ( ASTM standard)
Functions Dual-wavelength detection in the range 190 to 370 nm and upwards of 371, ratio-chromatogram output, wavelength scanning
Cell Optical wavelength: 10 mm, capacity: 12µ L, withstand pressure: 12 MPa
Cell temperature-control range 5oC above room temperature to 50oC
Web control Parameter setting, log management, monitor detector output, management of consumable parts, etc. Parameter setting, log management, management of consumable parts, etc.
Buffer memory Refer to CBM-20A/ Alite
Operating temperature range 4oC to 35oC
Dimensions, weight 260 ( W) x 140 ( H) x 420 ( D) mm, 13 kg
Power requirements 100 VAC, 160 VA, 50/ 60 Hz
For Research Use Only. Not for use in diagnostic procedures.
HPLC System Controller
The CBM-20A/ CBM-20Alite is the world' s first Web-based HPLC system controller. Equipped with a data buffering function, the CBM-20A/ CBM-20Alite acts as an interface for connecting LC workstations, network-client computers, and analytical instruments via Ethernet. An easy-to-use Web interface enables users to set-up, control and monitor their HPLC remotely. This centralized management ensures efficiency, offers added security with multi-level user access options, provides a flexible analytical environment, and helps to conserve valuable bench space.
Up to eight modules can be connected. Adding up to two A/ D conversion boards makes it possible to accept an analog signal from non-Shimadzu detectors.
The space-saving card-type CBM-20Alite is installed inside an LC-20A series pump or autosampler, and can control up to five modules, including the one in which it is installed.
Click the pictures to see enlarged CBM-20Alite screenshots
Flexible Installation Environment
Connected via LAN cables to the laboratory' s Ethernet, computers controlling the analytical instruments can be accessed remotely. The CBM' s data buffering function ( available only with LCsolution software) protects important analytical data from unexpected communications errors.
Integrated Web-Control Functions
In addition to an Ethernet connection, the CBM-20A/ CBM-20Alite is equipped with Web-control functions. These allow system control, monitoring, and maintenance information to be accessed from Internet Explorer without installing special software. Now, from a remote location, like your home or office, you can:
• Schedule maintenance for consumable parts
• Perform baseline check or system equilibration
• Monitor current analysis
• Save time by reducing visits to the laboratory to check on hardware
Auto-Purge with a Modular HPLC System
Equipped with auto-purge, user intervention is not required to open or close the drain valve. All pumps and autosamplers are purged on command or automatically. When combined with the system startup and baseline-check functions, fully automated analysis is achieved with a modular HPLC system.
Monitor Device Status at a Glance
With this function, the operating status of networked devices can be checked at a glance. You can monitor:
• System activity
• Status of individual units
• Existence of errors
• Device maintenance information
A memo function allows users to post messages regarding the status of components or to allow users sharing the system to leave messages for each other.
Click the pictures to see enlarged CBM-20A screenshots
Connectable Units Solvent delivery units: 4 max; Autosamplers: 1; Column ovens: 1; Detectors: 2 max; Fraction collectors: 1; Sub-controllers: 2 max. Solvent delivery units: 4 max; Autosamplers: 1; Column ovens: 1; Detectors: 2 max.
Number of connectable units 8 ( expansion possible up to 12) 5 ( including the unit incorporating the system controller)
Data Buffering Approx. 24 hours for one analysis
( at 500ms sampling rate; available only with LCsolution)
Event I/ O 4 inputs, 4 outputs 2 inputs, 2 outputs
Analog Boards Up to 2 boards can be mounted Mounting not supported
Operating temperature range 4° C to 35° C
Dimensions, weight 260 ( W) x 140 ( H) x 420 ( D) mm, 5.5 kg 120 ( W) x 20 ( H) x 100 ( D) mm, 0.5 kg
Power requirements 100 VAC, 100VA, 50/ 60HZ Supplied from unit
Dual Reciprocating Plunger Design
The LC-20AT possesses a high level of stability while maintaining high performance during routine use. The ability to remove pulsations has been improved by modifying the pump-head structure and the flow line.
Enhanced Solvent Delivery Specifications
Flow-rate accuracy and precision in the micro-flow rate region have been improved with the addition of a new type of check valve and modifications to the solvent delivery control method. Non-polar organic solvents such as hexane can be delivered stably.
New and improved plunger seal materials ensure robust, long-lasting performance. A sapphire plunger optimized according to its crystal structure and a precise plunger-holding mechanism help increase the service life of plunger seals and enable stable solvent delivery over long periods.
An optional low pressure gradient valve ( LC-20AD/ 20AT) , CBM-20Alite system controller, and a seal wash pump ( LC-20AD) can now be installed inside the solvent delivery unit, enabling 4-solvent gradient elution in a compact space. The LC-20AB high-pressure binary gradient pump delivers the performance of two LC-20AD pumps in one compact unit.
LC-20AD LC-20AT LC-20AB
Solvent delivery method Parallel-type double plunger Series-type double plunger Parallel-type double plunger ( 2 sets)
Plunger capacity 10µ L Primary side: 47µ L, Secondary side: 23µ L 10µ L
Maximum discharge pressure 40MPa
Flow-rate setting range 0.0001 to 10.0000 mL/ min. 0.001 to 10.000 mL/ min. 0.0001 to 10.0000 mL/ min.
Flow-rate accuracy No more than 1% or 0.5µ L/ min., whichever is greater ( 0.01 to 2 mL/ min.) No more than 2% or 2µ L/ min., whichever is greater ( 0.01 to 5 mL/ min.) No more than 1% or 0.5µ L/ min., whichever is greater ( 0.01 to 2 mL/ min.)
Flow-rate precision No more than 0.06% RSD or 0.02 min SD, whichever is greater
Pulsation 0.1 MPa ( for water at 1.0 mL/ min. and 7MPa) 0.3 MPa ( for water at 1.0 mL/ min. and 7MPa) 0.1 MPa ( for water at 1.0 mL/ min. and 7MPa)
Constant-pressure solvent delivery Supported
Plunger rinsing mechanism Manual rinsing or automatic rinsing using optional product
Safety measures Liquid-leakage sensor, high-pressure/ low-pressure limits
Operating temperature range 4oC to 35oC
Dimensions, weight 260 ( W) x 140 ( H) x 420 ( D) mm, 10 kg 260 ( W) x 140 ( H) x 420 ( D) mm, 11 kg 260 ( W) x 140 ( H) x 420 ( D) mm, 13 kg
Power requirements 100 VAC, 150 VA, 50/ 60 Hz 100 VAC, 180 VA, 50/ 60 Hz
LC-20AD/ 20AT, low-pressure GE specifications LC-20AD/ 20AT, high-pressure GE specifications LC-20AB
Gradient type Low-pressure mixing High-pressure mixing
Number of mixed solvents 4 max. 2 or 3 2
Mixing concentration accuracy 0.5% max. ( LC-20AD) , 1.0% max. ( LC-20AT) 0.5% max.
Rheodyne® Model 7125 Injector
The Rheodyne Model 7125 syringe loading injector allows injection of the entire contents of the syringe into the sample loop – no need to flush the valve between injections except for trace analyses. It also injects samples from a partially filled loop saving time by eliminating the need to change the sample loop.
The Model 7125 injector can be used at pressures up to 7000 psi ( 490 kg/ cm2) and is supplied with a VESPEL® rotor seal for operation at pH 0-10 ( use a Tefzel® seal at pH 0-14) . A 20 ¼ L sample loop is included; order additional loops separately.
When selecting replacement seals, use VESPEL seals to pH 10; Tefzel seals to pH 14.
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LabSolutions Series Workstation
Shimadzu' s LCsolution software offers an intuitive easy-to-use operating environment and sets a new standard for laboratory productivity in the 21st century by offering innovative solutions to meet the challenges of high sample throughput, instrument control, diverse data handling, and integration with regulatory compliance. Shimadzu Lab Solutions workstation unifies data acquisition and management across all types of chromatographic instruments. The operating environment for chromatography applications is standardized with the common user interface, which provides for improved productivity of your analysis.
Functionality and Operation
LCsolution' s assistant bar provides easy navigation between major modes of operation. Data explorer provides easy access to data, method and sequence files. By simply double-clicking a data file name, chromatogram and quantitation results can be viewed. Window layout can be saved with its name, which enables users to optimize their operation environment. Wizard functions enable easy parameter settings including peak integration, peak identification, and calibration curve information.
Automation for high productivity
Automation of analysis reduces the time spent on a variety of operations - from bringing the instrument to the optimal conditions before the run to the device shutdown.
For report generation, 26 types of report items are available, including sample information and chromatogram. You can modify existing templates or create your own for infinite possibilities.
OLE automation capability supports users´ customization programs. After the program is applied, the user can access data from custom software.
Reinforcement of 3-Dimensional Data Management
3-dimensional data analysis, which requires high functionality and operability, can use quantitative layouts, qualitative layouts, and custom layouts ( which can be saved with name and layouts) . Displaying necessary information, these layout screens can be switched easily. By the correlation of chromatogram and spectrum, peak-top spectra can be sequentially checked and a chromatogram at a designed wavelength can be displayed by double-clicking on the spectrum. In the spectrum index, spectra of detected peaks, including unidentified peak, can be viewed. 2 sets of SPD-M10AVP/ SPD-M20A can be controlled.
System administration includes the setting of system policy, user management, log browser and audit trails, which support 21 CFR Part 11 compliance.
Allows access to the system by system operation control, password validity, and its minimum length, which supports Part 11 compliance in both network and standalone environments.
Consists of the relations among groups and users. The permission pertains to a group and users gain permission by belonging to a group. User security and authorization management help optimize workflow for your laboratory.
Event logs regarding the log and system administration as well as the analytical instrument are displayed. When the user administration is shared with the Shimadzu User Authentication Tool, logs of the user authentication server are also displayed. An easy-to-use filter function quickly selects and displays only those records that match the set criteria.
The complete history of an instrument´ s operation and data processing parameters can be saved and output for traceability purposes. In addition, the data audit trail, data acquisition method, and the most recent analysis´ parameters are saved in the data file for future access. With LCsolution, it is also possible to apply audit trail functionality to system policy and as a global requirement for all methods created in the system.
Integrated Data Management
LCsolution has a function for automatic data transfer to CLASS-Agent database. With CLASS-Agent manager, data browsing, inspection, and approval of data for chromatography and spectrophotometry instruments and balances can be obtained without learning new operations for the workstation or analytical instrument' s software.